Human myeloid colony-producing potential in vitro of input cell fractions and of output cells from the BMs of repopulated mice. Total GM, erythroid (BFU-E), and GEMM colonies are plotted for 1 × 105 input cells from experiment 4 (•). BM cells from five mice that had each been infused with 1 × 105 input cells and allowed to engraft for 10 weeks were plated into the CFU assay. The means and SE of the total CFU in each category are plotted (○). All colonies analyzed by a human-specific PCR (at least 30 colonies/CFU type/mouse) were of human origin. Typical PCR signals are shown below specific colony types for human Cart-1 (lanes 6, 8, and 10) and for β-actin (lanes 7, 9, and 11). Controls consisted of PCRs for human Cart-1 (lanes 2 and 4) and β-actin (lanes 3 and 5) from human PB leukocytes (lanes 2 and 3) and normal mouse BM cells (lanes 4 and 5). Lane 1 shows a 123-bp ladder. The predicted amplified products for the hu Cart-1 PCR and for the β-actin PCR are 156 and 249 bp, respectively.
