α(1,2)FT activity directed by each of the wild-type H and five mutant H alleles. From left to right, pCDM8, activity of lysate of COS-1 cells mock-transfected with pCDM8 vector; pCDM8/H, activity of H-transfected COS-1 cell lysate; pCDM8/h1, h2, h3, h4 or h5, activity of h1, h2, h3, h4, or h5-transfected COS-1 cell lysate, respectively. The activity in each lysate was normalized by transfection efficiency, which was determined by expression of cotransfected luciferase expression vector. Data represent means and standard deviation (SD) of three independent assays.