Flow cytometric analysis of immunofluorescent staining of bone marrow cells from untreated and G-CSF–treated mice. (A) and (B) show the analyses of staining for Mac-1 versus TCRαβ receptors and Mac-1 versus B220 receptors in untreated mice. (D) and (E) show the analyses in treated mice. (C) shows the staining pattern of untreated cells for CD4 and CD8 versus TCRαβ receptors. Cells in (C) were gated for TCRαβ⁺ cells and staining for CD4 and CD8 versus NK1.1 receptors is shown in (F). The four boxes in (F) show the percentage of CD4⁺ or CD8⁺ NK1.1⁻ (1), CD4⁺ or CD8⁺ NK1.1⁺ (2), CD4⁻CD8⁻ NK1.1⁺ (3), and CD4⁻CD8⁻ NK1.1⁻ (4) αβ T cells.