Cytokine secretion patterns of blood mononuclear cells from untreated C57BL/Ka mice or from mice receiving G-CSF. (A), (B), and (C) show the concentration of IL-2, IFN-γ, or IL-4, respectively, in the supernatants from 0.2 × 10⁶ cells cultured for 48 hours in the presence of calcium ionophore and PMA. Each bar shows the average value of two or three replicate determinations of supernatants from one of three independent experiments: Ficoll-PBL (untreated mononuclear cells), Ficoll-PBC (G-CSF–treated mononuclear cells), and Percoll-LDC (G-CSF–treated low-density mononuclear cells). (D), (E), and (F) show the concentrations of IL-2, IFN-γ, or IL-4 from 0.2 × 10⁶ purified CD4⁺ and CD8⁺ T cells obtained by flow cytometry in two independent experiments: G⁻M⁻B⁻PBL (sorted Gr-1⁻ Mac-1⁻ B220⁻ mononuclear cells from untreated mice) and G⁻M⁻B⁻PBC (sorted Gr-1⁻ Mac-1⁻ B220⁻ mononuclear cells from treated mice).