Effects of IFN-γ and/or IL-6 on expression of p21 protein in patient MM cells. Total lysates from MM cells of patient no. 1 were prepared before (time 0) and after 2, 4, 8, 16, 24, 36, and 48 hours of culture with 10% FBS-RPMI-1640 media, IFN-γ (10 ng/mL), IL-6 (50 ng/mL), or IFN-γ (10 ng/mL) + IL-6 (50 ng/mL). Total cell lysates were immunoprecipitated with anti-p21 polyclonal Ab, followed by Western immunoblotting with anti-p21 MoAb.