Interphase and metaphase FISH using VH and Cγ gene. DAPI banding pictures are shown on the right side of the corresponding FISH metaphases except for (c). (a) Split pattern of VH (TRITC, red) and Cγ (FITC, green) gene signals in interphase nuclei of patient no. 45 with MGUS. (b) Metaphase FISH on patient no. 41 with t(11; 14). Signals of VH (red) and Cγ (green) genes are observed on 11q− and 14q+, respectively (arrows). An arrowhead indicates normal chromosome 14. (c) Metaphase FISH on patient no. 1 with t(8; 14). Through tripple-band-pass filter, signals of split VH (arrowhead, pink) and Cγ-split VH (double arrowhead, whitish pink) genes are observed on 8q− and 14q+, respectively. Normal chromosome 14 was labeled with whitish pink signal of the IgH gene (arrow). In this patient, the breakpoint of the IgH gene is possibly within the variable region. (d) Metaphase FISH on patient no. 20 with t(14; 18). Cγ (G, green) and VH (R, red) signals are observed on 14q+ and 18q−, respectively. Y (yellow) denotes a normal allele of the IgH gene. (e) Metaphase FISH on patient no. 11 with t(7; 14). Signals of VH (arrowheads) and Cγ (arrows) genes are observed on 7q− and 14q+, respectively. (f ) Metaphase FISH on patient no. 2 with complex 14q32.33 translocation involving 3q and 16q24. Signals of VH (R) and Cγ (G) genes are observed on 3q− and 16q, respectively. Y indicates normal chromosome 14.