Fig. 6.
Determination of the turnover time of the Ki-S2 antigen. L428 cells were labeled for 1 hour with [35S]methionine and intensively washed. Immunoprecipitation experiments were performed immediately after the end of labeling (A) and 90 minutes (B), 120 minutes (C), and 180 minutes (D) after the end of labeling. (E) Isotype control experiment after the end of labeling. Molecular weight standards are shown on the right (in kilodaltons).