Fig. 2.
Immunoprecipitates of biosynthetically labeled calreticulin from HL-60 cells. After 35S-methionine pulse labeling, the cells were disrupted and the labeled proteins immunoprecipitated with anticalreticulin. The precipitates were analyzed by 7.5% SDS-PAGE and fluorography. The locations of molecular weight markers are indicated. (A) Pulse labeling was performed for 15 to 120 minutes as indicated. (B) Pulse labeling was performed for 0 to 30 minutes as indicated. Long exposure of the film resulted in the appearance of unidentified lower molecular weight bands in the 30-minute pulse lane. (C) Pulse labeling was performed for 30 minutes and then followed by a chase with excess unlabeled methionine for 0 to 120 minutes as indicated.
