Effect of glycosidases on the electrophoretic mobility of calreticulin and myeloperoxidase synthesized in HL-60 cells. Biosynthesis using a 60-minute pulse label in HL-60 cells and immunoprecipitation with either anticalreticulin (CRT; lower panel) or antimyeloperoxidase (MPO; upper panel) were performed as in Figs 2 and 4. The immunoprecipitates were then incubated with Endo F buffer either alone (−/−) or plus the indicated amounts of endoglycosidase H (ENDO H) or N-glycosidase F (N-GLYC F ). The treated immunoprecipitates were then analyzed by 7.5% SDS-PAGE and fluorography.