Fig. 5.
![Fig. 5. PCR analysis of CD23 expression in SMS-SB and other B-lymphoid cell lines. Total cellular RNA was prepared from a range of B-lymphoid and keratinocyte cell lines, and from SMS-SB cultured either in PFHMII or in serum-containing medium. Total cellular RNA was used as template for RT-PCR using the primers indicated and the products were separated by agarose gel electrophoresis and probed with a [32P]-labeled insert from the plasmid pCDL SRα296CD23. Hybridization was visualized by autoradiography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.234/5/bl_0013f5.jpeg?Expires=1735547723&Signature=vllGbqAcndBuYCi5pO8B4AZnRzWYJlfw9IxQbEMmi79~7LaJZIbj4CMu1I743TmfriIgX-HfKIKO1GfM6L80NaEof57CswZ1LSeoKL4R6534VyV6hqNrCtQQqZDdQusPOzfnKAnCfk7E5b8p7TZM5M28bEIulxWaFBQV9OscVX3NUqo74u~hj8O72UIdAm34QaPWIyuwTObd24ztmazQql-RvxQbMpxIthif4-rJfNxZ0JAedzL70Fys4M74OLtdHhCUnXB7s4kMuyf88qhb4Sk3NvVADgrub~tsfaJ9nTbE2cBw5XMHo0O9X21D0i-4A0B0ej~73s4mZTGG4HHeGg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PCR analysis of CD23 expression in SMS-SB and other B-lymphoid cell lines. Total cellular RNA was prepared from a range of B-lymphoid and keratinocyte cell lines, and from SMS-SB cultured either in PFHMII or in serum-containing medium. Total cellular RNA was used as template for RT-PCR using the primers indicated and the products were separated by agarose gel electrophoresis and probed with a [32P]-labeled insert from the plasmid pCDL SRα296CD23. Hybridization was visualized by autoradiography.
