Fig. 2.
Tie receptor glycosylation. Cells were metabolically labeled with 35S-Translabel for 2 hours before preparation of cell lysates and immunoprecipitation with anti-tie MoAb 5D2 as described in Materials and Methods. Immunoprecipitates were treated with either buffer (MOCK), N-glycosidase (N-Gly), neuraminidase (Neur.), or a combination of O-glycosidase and neuraminidase (Neur. + O) for 20 hours at 37°C before SDS-PAGE and autoradiography. Molecular weight markers as indicated.
