Fig. 2.
Fig. 2. PCR amplification of Bcl-2 rearrangement at the Mbr. (A) Reverse view of an ethidium bromide-stained agarose gel. (B) Southern blot analysis of PCR products hybridized with an internal Mbr oligoprobe (see Materials and Methods). M, molecular weight marker V (Boehringer Mannheim, Meylan, France); W, negative control (water). Lanes 1 through 4, positive control diluted 10−5 to 10−2; lane 5, case no. 2; lane 6, case no. 13; lane 7, case no. 16; lanes 8 and 9, case no. 5, detection of a Bcl-2 rearrangement both in the bronchic MALT lymphoma (lane 8) and in the nodal large B-cell lymphoma (lane 9) occurring 2 years later.

PCR amplification of Bcl-2 rearrangement at the Mbr. (A) Reverse view of an ethidium bromide-stained agarose gel. (B) Southern blot analysis of PCR products hybridized with an internal Mbr oligoprobe (see Materials and Methods). M, molecular weight marker V (Boehringer Mannheim, Meylan, France); W, negative control (water). Lanes 1 through 4, positive control diluted 10−5 to 10−2; lane 5, case no. 2; lane 6, case no. 13; lane 7, case no. 16; lanes 8 and 9, case no. 5, detection of a Bcl-2 rearrangement both in the bronchic MALT lymphoma (lane 8) and in the nodal large B-cell lymphoma (lane 9) occurring 2 years later.

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