SSCP analysis of plasminogen gene exon 15 in patient 2, her healthy sister, and her parents. Radiolabeled single-stranded PCR products of plasminogen exon 15 were electrophoresed in a nondenaturing 5% polyacrylamide gel as described in Materials and Methods. Lane 1, healthy control (C); lane 2, patient 2 with ligneous conjunctivitis (II-1); lane 3, healthy mother of patient 2 (I-1); lane 4, healthy father of patient 2 (I-2); lane 5, healthy sister of patient 2 (II-2). dsDNA, double-stranded DNA.