Viability of FL5.12 clones expressing Flag-Bcl-2 or Flag-Bcl-XL after epipodophyllotoxin treatment. (A) Cells were seeded at 1 × 105 cells/mL in triplicate wells in media containing IL-3 and increasing concentrations of etoposide. Cells were lysed at 72 hours and analyzed by flow cytometry as described in Fig 2. A statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at 10 and 100 mg/mL of drug (P < .001, Student's t-test). (B) Cell viability was determined each time point following continuous incubation in media containing IL-3 and 10 μg/mL of etoposide. Cells were seeded at 1 × 105 cells/mL in triplicate wells and analyzed by flow cytometry as described in Fig 2. A statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at Days 3 and 4 (P < .001, Student's t-test). (C) Cells were seeded at 1 × 105 cells/mL in triplicate wells in media containing IL-3 and increasing concentrations of teniposide. Cells were lysed at 72 hours and analyzed by flow cytometry as described in Fig 2. A statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at 1, 10, and 100 μg/mL of drug (P < .001, Student's t-test). (D) Cell viability was determined as above at each time point following continuous incubation in media containing IL-3 and 10 μg/mL of teniposide. Cells were seeded at 1 × 105 cells/mL in triplicate wells and analyzed by flow cytometry as described in Fig 2. A statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at Days 2, 3, and 4 (P < .001, Student's t-test). Graphs shown in A, B, C, and D are the mean ± SD of triplicate cultures and representative of at least two separate experiments.
