Viability of FL5.12 clones expressing Flag-Bcl-2 or Flag-Bcl-XL after treatment with etoposide or methotrexate. (A) 1 × 105 cells were seeded in triplicate wells in media containing IL-3 and 10 mg/mL of etoposide. At each time point, cells were counted using trypan blue exclusion to assess viability. After 3 days of continuous incubation in etoposide, the cells were washed twice and replated in triplicate at the viable cell number for Day 3 in media containing IL-3. Arrow indicates time point at which the cells were washed and replated in drug-free media containing IL-3. A statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at each time point after Day 1 (P < .01, Student's t-test). (B) 1 × 105 cells were seeded in triplicate wells in media containing IL-3 and 1.0 mg/mL of methotrexate. At each time point, cells were counted using trypan blue exclusion to assess viability. After 3 days of continuous incubation in methotrexate, the cells were washed twice and replated in triplicate at the viable cell number for Day 3 in media containing IL-3. Arrow indicates time point at which the cells were washed and replated in drug-free media containing IL-3. A statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at each time point after Day 1 (P < .01, Student's t-test).
