Fig. 3.
![Fig. 3. Inhibitory effect of PAR3 IgG on α-thrombin–induced calcium mobilization in mouse platelets. Mouse platelets were loaded with fura-2 and cytosolic free Ca2+ concentration [Ca2+]i (nmol/L) was measured fluorometrically.14 Extracellular calcium was chelated to prevent platelet aggregation. Platelets were preincubated with the indicated IgG preparations for 5 minutes at 37°C and then transferred to a fluorometer at room temperature. α-Thrombin (0.5 nmol/L) or U46619 (3 μmol/L) was added at approximately 100 seconds. The data shown are representative of three experiments with thrombin and two experiments with U46619.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/11/10.1182_blood.v91.11.4152/4/blod41122003x.jpeg?Expires=1735833580&Signature=bctuFTvuPNZ6kGsegiu~N5flOW3GnOkB8jBS9rhqkZAGRya48NHW56k51dw0HlRDrCENh-BYFpbGm~YfZ8GIqvfF0nRsdd9dDOV-IoiLZjkBdBJkmgxAHSzCSzATOLd7dTr4Gmm7K6WrBHdmr~BZLtmdfJ5UrzJenbgJZfVuFV~xvsYwy~4IoKtq5sK-M1rV96NpyQ-1HOUQGX9IvSVxu6nV6Rk8xwkqZvFcDSyUPylcvg-sNLyPIVK--salLfzQicCStb7OsXo7gp-P8cXteyZDC1sg5mrZTx4w4N~sztG2zyTNeBryWTpOd4~s2ZjrJMEQL-JAmaJooAZp6~Bu6g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibitory effect of PAR3 IgG on α-thrombin–induced calcium mobilization in mouse platelets. Mouse platelets were loaded with fura-2 and cytosolic free Ca2+ concentration [Ca2+]i (nmol/L) was measured fluorometrically.14 Extracellular calcium was chelated to prevent platelet aggregation. Platelets were preincubated with the indicated IgG preparations for 5 minutes at 37°C and then transferred to a fluorometer at room temperature. α-Thrombin (0.5 nmol/L) or U46619 (3 μmol/L) was added at approximately 100 seconds. The data shown are representative of three experiments with thrombin and two experiments with U46619.
