Localization of TGF-α in monocytes after incubation with IL-10 and IFN-γ for 24 hours and labeled for TGF-α. Monocytes are polarized and degranulated. Still some granules are observed mainly underneath the cell surface, where the cell membrane is ruffled (large arrows). The granules are labeled (small arrows and inset). Inset is a higher magnification of the labeled area with asterisk. Bars = 500 nm, inset = 100 nm.