Effect of Jagged coated beads on purified hematopoietic precursors. Enriched mouse marrow cells (Lin⁻sca-1⁺c-kit⁺) were added to wells containing cytokines (IL-11, IL-6, SCF, and Flt3-ligand) and beads coated with Jagged-1^ext (▪) or control beads (□). After 7 days of incubation cells were harvested and replated in semisolid medium containing growth factors. After 10 days of incubation the colony types were quantitated and a mean number of colonies per well was derived from each experiment. Each bar represents the mean number of HPP-mix (A) or CFU-C (B) colonies per well from five separate experiments ± SEM. HPP-mix refers to colonies larger than 1.5 mm in diameter and consisting of multiple lineages including at least granulocyte/macrophage and erythroid clusters.