Effect of Jagged-1^ext coated beads on the time course of formation of clonogenic cells. Two hundred enriched mouse marrow cells (Lin⁻sca-1⁺c-kit⁺) were added to wells containing cytokines (IL-11, IL-6, SCF, and Flt3-ligand) and beads coated with Jagged-1^ext (□) or control beads (○). Cells were removed at time intervals and replated in semi-solid medium. After 10 days the total number of cells per 200 starting cells (A), HPP-mix (B), and CFU-C (C) were determined for each well. Each point represents the mean from 8 wells in experiment 1 and 10 wells in experiment 2.