Electrophoretic analyses of ferritin purified from HHCS lens. (A) SDS-PAGE of the purified ferritin compared with human H and L ferritin chains, Coomassie blue stain. (B) Nondenaturing PAGE of the purified lens ferritin compared with recombinant H and L ferritins, Coomassie blue stain. (C) The same as in (B), except that ferritins were preincubated with Fe(II) (1,000 Fe atoms per molecule, pH 7.0) and the gels stained with Prussian Blue.