Fig. 5.
Fig. 5. Western blots of murine factor XI. (A) Murine factor XI activation by murine factor XIa in the presence of dextran sulfate. Murine factor XI (5 μg/mL) was incubated with 1 μg/mL dextran sulfate and murine factor XIa (5 nmol/L) at 37°C. (B) Murine factor XI autoactivation. Murine factor XI (5 μg/mL) was incubated with 1 μg/mL dextran sulfate at 37°C. (C) Murine factor XIa (5 μg/mL) was incubated with 1 μg/mL dextran sulfate at 37°C. Western blots were prepared for all experiments as described in Materials and Methods. The positions of molecular weight markers in kD are shown at the left of the figure. Abbreviations: fXI, factor XI zymogen; fXIc, factor XI cleaved; HC, factor XIa heavy chain; LC, factor XIa light chain; and C, cleavage product-possibly of the light chain. The sample in lane XIa is an activated murine factor XI standard prepared by incubating factor XI with factor XIIa in the absence of dextran sulfate.

Western blots of murine factor XI. (A) Murine factor XI activation by murine factor XIa in the presence of dextran sulfate. Murine factor XI (5 μg/mL) was incubated with 1 μg/mL dextran sulfate and murine factor XIa (5 nmol/L) at 37°C. (B) Murine factor XI autoactivation. Murine factor XI (5 μg/mL) was incubated with 1 μg/mL dextran sulfate at 37°C. (C) Murine factor XIa (5 μg/mL) was incubated with 1 μg/mL dextran sulfate at 37°C. Western blots were prepared for all experiments as described in Materials and Methods. The positions of molecular weight markers in kD are shown at the left of the figure. Abbreviations: fXI, factor XI zymogen; fXIc, factor XI cleaved; HC, factor XIa heavy chain; LC, factor XIa light chain; and C, cleavage product-possibly of the light chain. The sample in lane XIa is an activated murine factor XI standard prepared by incubating factor XI with factor XIIa in the absence of dextran sulfate.

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