American Society of Hematology

Fig. 6.

$Fig. 6. Inhibition of rHFV des B binding to phosphatidylserine by IgG fractions. Recombinant factor V des B was incubated with the IgG fractions at the concentrations shown for 15 minutes at 37°C, and then incubated in the PS-coated wells as described in Materials and Methods. Binding to PS was determined by ELISA, as previously described.18 The IgG fractions shown are: H1 (▪); H3 (□); RS (⧫); RB (◊); WH (•); AR (○); EM (▴); RH (▵); and H4 (). Controls include the IgG fraction from pooled normal plasma (⊕) and the murine MoAb HV-1 (⊠).$

Inhibition of rHFV des B binding to phosphatidylserine by IgG fractions. Recombinant factor V des B was incubated with the IgG fractions at the concentrations shown for 15 minutes at 37°C, and then incubated in the PS-coated wells as described in Materials and Methods. Binding to PS was determined by ELISA, as previously described.18 The IgG fractions shown are: H1 (▪); H3 (□); RS (⧫); RB (◊); WH (•); AR (○); EM (▴); RH (▵); and H4 (). Controls include the IgG fraction from pooled normal plasma (⊕) and the murine MoAb HV-1 (⊠).

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