IL-10 is not an intermediate in the suppression of IL-12 by GC. Cytokines were induced in whole-blood cultures as described in the legend of Fig 1. Cultures were performed in the absence or presence of 5 μg/mL anti–IL-10 with or without 10−6 mol/L DEX (top panel). Results are expressed as a percentage ± SEM of the cytokine production found in the presence of LPS and in the absence of anti–IL-10. The results are the means of the IL-12(p40) (▪) and TNF-α (▨) production obtained with nine different healthy donors; IL-12(p70) production (▩) is based on results obtained with four different healthy donors. In additional experiments, the effect of 50 μmol/L RU486 (▨), 5 μg/mL anti-IL-10 (▩), or both () on LPS– and IFN-γ–induced IL-12(p70) was studied in nine separate donors (bottom panel). The results are expressed as a percentage ± SEM of the cytokine production in the presence of LPS and IFN-γ alone (▪).
