Effect of shear stress on TF antigen levels in cell lysate. (A) HUVECs were exposed to shear stress (18 dynes/cm2) or left static for 15 hours before and 1, 3, 6, and 9 hours after TNF-α (100 U/mL) addition, and TF antigen levels in cell lysate were measured by ELISA methods. Data represent mean ± SD of three separate experiments. *, Significantly different from static control at the same time points (P < .05, Student's t-test). **, Significantly different from static control at the same time points (P < .01, Student'st-test). (□), 0 dyne/cm2; (•), 18 dynes/cm2. (B) HUVECs were exposed to shear stress (6, 12, 18, and 24 dynes/cm2) or left static for 15 hours before and 6 hours after TNF-α (100 U/mL) addition, and TF antigen levels in cell lysate were measured by ELISA methods. Data represent mean ± SD of three separate experiments. ***, Significantly different from static control treated with TNF-α (P < .01, ANOVA-Dunnett test).
