Fig. 4.
Effect of shear stress on TF antigen levels on cell surface of HUVECs. HUVECs were exposed to shear stress (18 dynes/cm2) or left static for 15 hours before and 6 hours after TNF-α (100 U/mL) addition, and TF antigen levels on the cell surface were analyzed by flow cytometry using antihuman TF monoclonal antibody, HTF-K180. Representative plots from three separate experiments with essentially the same results. Profile A, nontreated cells; profile B, TNF-α–treated cells; profile C, presheared and sheared TNF-α–treated cells.
