Effect of shear stress on TF procoagulant activity of HUVECs. HUVECs were exposed to shear stress (18 dynes/cm2) or left static for 15 hours before and 1, 3, 6, and 9 hours after TNF-α (100 U/mL) addition. HUVECs were also exposed to shear stress itself (18 dynes/cm2) for 1, 3, 6, and 9 hours or left static. In four different conditions, TF procoagulant activity was measured by chromogenic assay as assessed by factor Xa generation on monolayers of HUVECs. Data represent mean ± standard error (SE) of three separate experiments from TNF-α (100 U/mL)–stimulated cells and four separate experiments from nonstimulated cells. (•), TNF-stimulated cells; (□), presheared and sheared TNF-stimulated cells; (▪), sheared, nonstimulated cells; (○), static, nonstimulated cells.
