Fig. 6.
Effect of treatment with ASA or L-NAME on shear-induced attenuation in TF mRNA level of HUVECs. (A) After pretreatment with ASA (1 mmol/L) for 30 minutes, HUVECs were exposed to shear stress (18 dynes/cm2) or left static for 15 hours before and 1 and 3 hours after TNF-α (100 U/mL) addition, and then processed as in Fig1. (B) HUVECs were exposed to shear stress (18 dynes/cm2) or left static for 15 hours before and 1 and 3 hours after TNF-α (100 U/mL) addition in the presence of L-NAME (100 μmol/L), and then processed as in Fig 1. Results of densitometric analysis of TF RT-PCR products normalized with respect to corresponding GAPDH RT-PCR product levels are shown.
