CDR3 size profiles of TCR Vβ5 and TCR Vβ 17 transcripts in samples from patient Cou analyzed with fluorescent Cβ, Jβ2.3, and Jβ2.7. cDNA made from total RNA extracted from TC5 (Vβ5) and TC7 (Vβ17) CTL clones, TIL-culture, tumor suspensions, and PBL from the patient were amplified in a PCR reaction primed by Vβ5/Vβ17– and Cβ–specific primers. The unlabeled amplification products were elongated with nested fluorescent Cβ, Jβ2.3, and Jβ2.7. Aliquots were subjected to electrophoresis and analysis on an automated sequencer. Arrows indicate the signal corresponding to the two CTL clones TCR transcripts.