BCL2 protein expression in diffuse large B-cell lymphoma. A Western immunoblot analysis was performed using a BCL2-specific monoclonal antibody. Lymphoma with t(14; 18) translocation was used as a positive control (P) and cases with loss of 18 (gels I and II) and normal 18 (gel III) were used as negative controls (N). The samples loaded to each gel are marked on the top of the figures. Protein from reactive lymphatic tissue (RL) was added to two gels (I and III).