Fig. 2.
Fig. 2. Phenotype of TCR Vβ8.1 DP TG thymocytes during in vitroMtv-7 SAg-mediated negative selection. Dot plots show the CD4 versus CD8 profiles (CD4-PE/CD8-FITC staining) of TCR Vβ8.1 TG thymocytes cultured for 36 or 60 hours alone or with BALB.D2 splenic B cells. The absolute cell number of DP and SP thymocytes is indicated. Circles indicate the DP CD4+CD8intpopulation. Histograms represent the FSC, CD69 expression, TUNEL staining, and BrdU incorporation of DP thymocytes for each culture condition. Staining details are given in Materials and Methods. The percentage of CD69+ (FL2 > 20), blast (FSC > 70), TUNEL+ (FL1 > 300), and BrdU+(FL1 > 130) DP thymocytes are indicated. The experiment is representative of four experiments with similar results. (Note that the CD4-TC/CD8-FITC staining used in Fig 1 dot plots does not allow a clear definition of the CD4+CD8int DP population.)

Phenotype of TCR Vβ8.1 DP TG thymocytes during in vitroMtv-7 SAg-mediated negative selection. Dot plots show the CD4 versus CD8 profiles (CD4-PE/CD8-FITC staining) of TCR Vβ8.1 TG thymocytes cultured for 36 or 60 hours alone or with BALB.D2 splenic B cells. The absolute cell number of DP and SP thymocytes is indicated. Circles indicate the DP CD4+CD8intpopulation. Histograms represent the FSC, CD69 expression, TUNEL staining, and BrdU incorporation of DP thymocytes for each culture condition. Staining details are given in Materials and Methods. The percentage of CD69+ (FL2 > 20), blast (FSC > 70), TUNEL+ (FL1 > 300), and BrdU+(FL1 > 130) DP thymocytes are indicated. The experiment is representative of four experiments with similar results. (Note that the CD4-TC/CD8-FITC staining used in Fig 1 dot plots does not allow a clear definition of the CD4+CD8int DP population.)

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