Fig. 2.
![Fig. 2. In vitro p38 activity is induced by Epo and IL-3 stimulation. FD-EPO cells (A) and FDC-P2 cells (B and C) were stimulated with Epo (A) and IL-3 (B), respectively, for the indicated time up to 60 minutes or stimulated with (+) or without (−) NaCl (C) for 30 minutes. The immunoprecipitates with anti-p38–specific antibody were incubated with [γ-32P]ATP and GST-ATF-2 as a substrate. Arrows indicate the phosphorylated GST-ATF-2 (molecular weight, 40 kD).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.929/4/bl_0066f2.jpeg?Expires=1735547783&Signature=DqbdccgUw9h-Y0hOXPgnxz5U1M0GnJsxWmusIr3o~Y~RaNOPBDWb3NquX535CX2Xx1XwhRDLfT4UPFgI4e8Wb6VBKHx5M33~e21lXZBY1B~SkRXvI0REEu8dtKcLDYYrOs8DIeehUc836B5uWf9~FWUulnmCVGWubpCGl52q73gzociqxQqXc9~HM3J7MdFGvMDBShWM70Dx6Fx3GGdMpRvMvJyL7wSRPuwyFeHp9OmEVxkyVq0bnr~Y~09oy6xuih81GS5BDAibWnXeEasA0hzH9GPbS69flSTHrbvtnucpiGphk51J3pB-Q2SDkQIFXLjwdt8-lc--U1Hg0lfRUA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro p38 activity is induced by Epo and IL-3 stimulation. FD-EPO cells (A) and FDC-P2 cells (B and C) were stimulated with Epo (A) and IL-3 (B), respectively, for the indicated time up to 60 minutes or stimulated with (+) or without (−) NaCl (C) for 30 minutes. The immunoprecipitates with anti-p38–specific antibody were incubated with [γ-32P]ATP and GST-ATF-2 as a substrate. Arrows indicate the phosphorylated GST-ATF-2 (molecular weight, 40 kD).
