Fig. 3.
![Fig. 3. In vitro MKK6 assay. FD-EPO cells (A) and FDC-P2 cells (B and C) were stimulated with Epo (A) and IL-3 (B), respectively, for the indicated time up to 60 minutes or stimulated with (+) or without (−) NaCl (C) for 30 minutes. MKK6 activity was measured in the immunoprecipitates with antispecific MKK6 antibody in the presence of [γ-32P]ATP and His-p38 as a substrate. Lane C, the kinase assay was performed without immunoprecipitates (only His-p38). Arrows indicate the phosphorylated His-p38.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.929/4/bl_0066f3.jpeg?Expires=1735548498&Signature=OygAxQK19aL9i2~4jlw-MpYjiWG8DkYcaliNflExylKHAxml~83kQ5ZAFbx9eexpUj0yc6Lm0PBUJTVo2t-oNyM2QwjL5X3~lloBMeYGJo0RJl4gXJkFaEubsLhI5VYW3cCeDdHwV28~8VJt6CvFZ3pIPI~PSX4gdG61fcsm2F5vFKQL1TH7tksIOXTiIZMGXUrY6oK3iMMzSB3x0sMA4p1h0RI94GjhIX5rraq1ouVoYatNFAeYA8AF5XeU41f9A7SE1ihQtibcPYMoR7ghGO5f0CJbMpxxbMbwzAPAtsKHYd07ak~YJgxH1joa1NsdhzzBH4Yj6yhHhZZ41YAxIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro MKK6 assay. FD-EPO cells (A) and FDC-P2 cells (B and C) were stimulated with Epo (A) and IL-3 (B), respectively, for the indicated time up to 60 minutes or stimulated with (+) or without (−) NaCl (C) for 30 minutes. MKK6 activity was measured in the immunoprecipitates with antispecific MKK6 antibody in the presence of [γ-32P]ATP and His-p38 as a substrate. Lane C, the kinase assay was performed without immunoprecipitates (only His-p38). Arrows indicate the phosphorylated His-p38.
