Fig. 4.
![Fig. 4. In vitro MKK3/MKK6 assay. FD-EPO cells (A) and FDC-P2 cells (B and C) were stimulated with Epo (A) and IL-3 (B), respectively, for the indicated time up to 60 minutes or stimulated with (+) or without (−) NaCl (C) for 30 minutes. The immunoprecipitates with antiphosphorylated MKK3/MKK6 antibody were incubated in the presence of [γ-32P] ATP and His-p38 as a substrate. Lane C, the kinase assay was performed without immunoprecipitates (only His-p38). Arrows indicate the phosphorylated His-p38.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.929/4/bl_0066f4.jpeg?Expires=1735548446&Signature=mYHznbPz~WNoCi8obZMSiRk0fdeQy1UWbIwhCA3IXcV3QqFpNwcmyv~W058OZBOzpvObs2P5wgFAre6GA9pIcl75VM-ctAMePOfv7q1ntgszdVag2QME2bEtyi7xB0eHjqp415GwA1wtWA9xgLQT0t65M~Nu1e4eGd5key2Y91DCOBCHb0OJtvfnyaVYN005iUJYSgNQc~BybMTDCKbRwRGt6d8bweBnAw-cJI5kH0sQumC38KlNrMcwRTam8aCg9EYmdylJU8YsnNEhKEpy1-y4yUMkfs9nrHmYoDRIKUvMkR2S8AhV2sI1PfVB3Pt3NRBI2PNlyYggtovAsY6IMw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro MKK3/MKK6 assay. FD-EPO cells (A) and FDC-P2 cells (B and C) were stimulated with Epo (A) and IL-3 (B), respectively, for the indicated time up to 60 minutes or stimulated with (+) or without (−) NaCl (C) for 30 minutes. The immunoprecipitates with antiphosphorylated MKK3/MKK6 antibody were incubated in the presence of [γ-32P] ATP and His-p38 as a substrate. Lane C, the kinase assay was performed without immunoprecipitates (only His-p38). Arrows indicate the phosphorylated His-p38.
