Effect of TN-C–added LTBMCs on colony formation by the produced cells from LTBMCs of TN-C–deficient mice and of control mice. At weeks 3 and 6 after induction of LTBMCs with TN-C at the final concentration of 0, 4, 20, and 100 ng/mL, nonadherent cells were obtained and cultured in methylcellulose medium as described in Materials and Methods. Colonies (>50 cells) were counted at day 14. Visualized hemoglobinized colonies were counted as erythroid bursts (BFU-E). Results are mean ± SD of triplicate plates and are expressed as the percentage of the value obtained in nonadherent cells from control LTBMCs without TN-C, which formed 134 ± 4 colonies including 14 ± 1 erythroid bursts per 5 × 104 nonadherent cells at week 3, and 103 ± 7 colonies including 11 ± 1 erythroid bursts at week 6 of LTBMCs. TN-C DM, tenascin-C–deficient mutant mice.
