American Society of Hematology

Fig. 1.

$Fig. 1. Size exclusion HPLC analysis of cytosolic (A) and nuclear (B) extracts prepared from fresh blasts from the PB of an APL patient expressing the bcr3-PML/RARα isoform. Extracts (200 μL) were incubated with 10 nmol/L [3H]-t-RA in the absence (•) or in the presence (○) of 200-fold mol/L excess of unlabeled t-RA for 18 hours at 4°C. The samples were then fractionated over a Superose 6 HR 10/30 size exclusion column as described in Materials and Methods.$

Size exclusion HPLC analysis of cytosolic (A) and nuclear (B) extracts prepared from fresh blasts from the PB of an APL patient expressing the bcr3-PML/RARα isoform. Extracts (200 μL) were incubated with 10 nmol/L [³H]-t-RA in the absence (•) or in the presence (○) of 200-fold mol/L excess of unlabeled t-RA for 18 hours at 4°C. The samples were then fractionated over a Superose 6 HR 10/30 size exclusion column as described in Materials and Methods.

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