Fig. 5.
![Fig. 5. Size exclusion HPLC analysis of [3H]-t-RA binding to nuclear (A) and cytosolic (B) extracts prepared from pMT2/PLZF/RARα transiently transfected COS-1 cells. (•) [3H]-t-RA alone; (○) [3H]-t-RA plus 200-fold mol/L excess of unlabeled t-RA. The Superose 6 HR 10/30 size exclusion column was calibrated with the indicated marker proteins (in thousands) as described in Materials and Methods.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.1175/4/bl_0029f5.jpeg?Expires=1735504345&Signature=48MCJX5bzj8ZBvW2SfHv0gFTvBJ9E53S1q0uYocCca~-OU~giOBXDcOR~Q7YRUlo5z-tI5fzm1YDrXARzGtSLx5slN2FZMTa7udQgcUI08yTzK0lEpfXjHmSnERVKMijZY8oPwj0uOh0rNIqdbR8OcNOX5B~hNpjSqT1H78uO-4JhzOHQ6C~MQ42z8YCsAvIa3jwNRwDKbkXACJk87YC1C2FnOoLzdweDlY2sKUf6~a3l7~y5wubCkP4GCH8bPJh~0C29xu7IA90Tnk74AtXWybWewf-KTHGNRTzSRkPozwsshSZNtc7EsV1aT8mFPgiwFzfRdrxUkZ~MZRoEKt8Hw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Size exclusion HPLC analysis of [3H]-t-RA binding to nuclear (A) and cytosolic (B) extracts prepared from pMT2/PLZF/RARα transiently transfected COS-1 cells. (•) [3H]-t-RA alone; (○) [3H]-t-RA plus 200-fold mol/L excess of unlabeled t-RA. The Superose 6 HR 10/30 size exclusion column was calibrated with the indicated marker proteins (in thousands) as described in Materials and Methods.
