American Society of Hematology

Fig. 2.

$Fig. 2. Effect of bone marrow donor age on efflux of Rh-123. Thy-1.1lowSca-1+Lin− HSC and normal unfractionated bone marrow isolated from groups of mice of the indicated ages were stained with Rh-123 as described in Materials and Methods. (A) Shaded profiles indicate Rh-123 fluorescence in normal bone marrow cells, while solid profiles show fluorescence of Thy-1.1lowSca-1+Lin− HSC isolated from the same bone marrow preparation. (B) Solid lines indicate the Rh-123 fluorescence intensity of Thy-1.1lowSca-1+Lin− HSC isolated from 10-month or 3-week-old mice, except in the indicated panel where the solid line indicates total bone marrow cells. Stippled profiles indicate the same cells stained with Rh-123 in the presence of 20 μg/mL cyclosporin A; similar results were obtained using verapamil as an MDR modulator (data not shown).$

Effect of bone marrow donor age on efflux of Rh-123. Thy-1.1^lowSca-1⁺Lin⁻ HSC and normal unfractionated bone marrow isolated from groups of mice of the indicated ages were stained with Rh-123 as described in Materials and Methods. (A) Shaded profiles indicate Rh-123 fluorescence in normal bone marrow cells, while solid profiles show fluorescence of Thy-1.1^lowSca-1⁺Lin⁻ HSC isolated from the same bone marrow preparation. (B) Solid lines indicate the Rh-123 fluorescence intensity of Thy-1.1^lowSca-1⁺Lin⁻ HSC isolated from 10-month or 3-week-old mice, except in the indicated panel where the solid line indicates total bone marrow cells. Stippled profiles indicate the same cells stained with Rh-123 in the presence of 20 μg/mL cyclosporin A; similar results were obtained using verapamil as an MDR modulator (data not shown).

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