Southern blot analysis of the genomic DNAs prepared from the leg muscle tissues of tumor-bearing animal. Aliquots of the total genomic DNA samples (10 μg) prepared from the leg tissues of a tumor-bearing animal were digested with BamHI, and subjected to Southern blot analysis (see the Materials and Methods). The unique human factor IX transgene bands detected (2.9 kb in size as marked on the right side) were quantified with PhosphorImager. Lane 1, DNA from the right hind leg with tumor; lane 2, DNA from the left leg with no tumor; lane 3, control DNA of primary myoblasts transduced with BAG (β-galactosidase retrovirus).