American Society of Hematology

Fig. 2.

$Fig. 2. GM-CSF enhances p52 nuclear translocation in TF-1 cells. A total of 1 × 107 TF-1 cells stimulated with GM-CSF for 0 to 24 hours were used for nuclear or cytoplasmic fractionation. A total of 15 μg of nuclear or cytosolic protein was loaded on each lane of a 10% polyacrylamide gel and subjected to Western blotting with anti-p50, anti-p52, anti-p65, or anti-IκBα antibodies and ECL. A representative blot of four independent experiments is shown.$

GM-CSF enhances p52 nuclear translocation in TF-1 cells. A total of 1 × 10⁷ TF-1 cells stimulated with GM-CSF for 0 to 24 hours were used for nuclear or cytoplasmic fractionation. A total of 15 μg of nuclear or cytosolic protein was loaded on each lane of a 10% polyacrylamide gel and subjected to Western blotting with anti-p50, anti-p52, anti-p65, or anti-IκBα antibodies and ECL. A representative blot of four independent experiments is shown.

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