Fig. 6.
EMSA of nuclear extracts from day 10 BFU-E–derived cells. A total of 6 μg of nuclear extracts from day 10 BFU-E–derived cells was incubated with different NF-κB antibodies for 10 minutes before adding 32P-labeled c-myb or c-mycκB binding oligonucleotide probes. Two DNA protein complexes were generated. Complex 1 and 2 (C1 and C2) were supershifted by anti-p50 and anti-p52; C1 was supershifted by anti-p65. Anti–Bcl-3 antibody had a minimal effect on these complexes. Three experiments were performed with similar results.
