Ribozyme-mediated inactivation of GRP94 causes severe reduction in the level of FAC. (A) NRK-Vect and NRK-Ribo cells transfected with pED6 (mock) or pED6-FAC (FAC) were either untreated or treated with 100 nmol/L MMC for 24 hours. Cytosolic lysates were then assayed for expression of FAC and GRP94 simultaneously by immunoblotting. (B) The same immunoblot as in Fig 3A was reprobed with an antibody against β-tubulin.