In situ appearance and the constituent cells of SCF + GM-CSF–dependent or SCF + GM-CSF + TPO–dependent colonies. (A) and (C) show the colonies with the maximal size grown by stimulation with SCF + GM-CSF or with SCF + GM-CSF + TPO from JCML CD34⁺CD38^high cells, respectively. (B) and (D) show the staining of the constituent cells of 20 pooled colonies grown by SCF + GM-CSF or with SCF + GM-CSF + TPO from JCML CD34⁺CD38^high cells with both ANB (brown) and NASDCA (blue), respectively. (E) and (F) show the staining of the constituent cells of 20 pooled colonies grown by SCF + GM-CSF + TPO from normal or JCML BM CD34⁺CD38^high cells with an MoAb for CD41b using the LSAB Kit, respectively.