Fig. 3.
Effect of cytokines on the expression of PGI2-R in HEL and NS-Meg cells. At the initiation of culture, either IL-3, IL-6, GM-CSF, TPO, IL-1β, or TNF-α was added to the culture and cells harvested at the indicated times were examined by Northern analysis. The controls were the cells cultured without cytokines for the respective culture periods (data not shown, similar to the controls for Fig 2). The figure shows representative results from triplicate experiments repeated twice. (A) All cytokines upregulated the expression of PGI2-R mRNA. (B) Effect of TPO on the expression of PGI2-R, PF4, GPIIb mRNA in HEL and NS-Meg cells. TPO increased the expression of PGI2-R, PF4, and GPIIb mRNA in HEL cells, and PGI2-R and GPIIb in NS-Meg cells.
