Fig. 6.
![Fig. 6. Effect of PMA and TPO on PGI2-R expression. HEL () and NS-Meg (▪) cells were preincubated for 48 hours with or without (control) either PMA or TPO. PGI2-R expression was evaluated by specific [3H]iloprost binding to the cells. Values (mean ± SE, n = 4) are expressed as a ratio of scintillation count of the control cells (nontreated HEL, 1.3 × 105 ± 5.3 × 103 dpm/1 × 106 cells, nontreated NS-Meg, 4.2 × 104 ± 3.9 × 103). Preincubation with PMA or TPO increased the binding capacity of the ligand up to sixfold and threefold in HEL cells and fivefold and twofold in NS-Meg cells, respectively, when compared with control values. The experiment is representative of three performed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.1039/4/bl_0006f6.jpeg?Expires=1735516640&Signature=M~urwUW-gALQ-3MlO9pvvaz1CihCDqZJ7HN8MZgf0ONc-Au3UkB9rLdM63R7b3NTUpMgaBFP-aIbng7Wiz44pNPvabA2zO19kf1i~ov2~cw6iP9LJCWCp5VmjXHuSSt0mEc~0Zu-jRTLTDdW7fcGjeNFdcUYizUFDMKsb9Ev97QLxp9VFZd5dXJRP33FnFtiAeXMcgM3r3C~MXhPBrsp8O7bB~U4mn0wco2tz-t0xFF9tfjLjid4sYkXMNUolWpIgdMCNKpomncKLPXOjII3Nb1i5aWwkeovdsk7c5EuishPxJvMgzsg5G-4cPpRJ34By4856DfV7V0cHVd-lmctQQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of PMA and TPO on PGI2-R expression. HEL () and NS-Meg (▪) cells were preincubated for 48 hours with or without (control) either PMA or TPO. PGI2-R expression was evaluated by specific [3H]iloprost binding to the cells. Values (mean ± SE, n = 4) are expressed as a ratio of scintillation count of the control cells (nontreated HEL, 1.3 × 105 ± 5.3 × 103 dpm/1 × 106 cells, nontreated NS-Meg, 4.2 × 104 ± 3.9 × 103). Preincubation with PMA or TPO increased the binding capacity of the ligand up to sixfold and threefold in HEL cells and fivefold and twofold in NS-Meg cells, respectively, when compared with control values. The experiment is representative of three performed.
