Fig. 8.
![Fig. 8. Effect of thrombin on CD45 and Lck reconstituted deficient clones. (A) Thrombin-induced [Ca2+]i responses. JE6.1 (□), J45.01 (⊞), J45.01/CD45 (⧫), JCaM1 (▴), or JCaM1/Lck (◊) were stimulated with 100 nmol/L thrombin and fluorescence analyzed as a function of time. (B and C) Thrombin-mediated phosphorylation of p38MAPK. Cells were stimulated with 100 nmol/L thrombin for the times indicated. Proteins from cell lysates were subjected to SDS-PAGE as described in the legend to Fig 4 and were transferred to Immobilon membranes for Western blotting using phospho-specific p38 MAPK antibody. Equal amounts of p38 MAPK were detected in each condition (not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/11/10.1182_blood.v91.11.4232/4/blod41132008w.jpeg?Expires=1735835386&Signature=Tb6DyVkvuucmaSRlqVEBEV4v7keIMfMF04Vtfw1OkgVxu6fWWdULWCboDDSdEFspDGObOsfp8pxhmGaVOFY~5avnlz60uJRJ0TdUG04ZvkfYS9TebJ8e9AoOdJswYPtZEfNkDif82aXLoTKw3zH7cPg2VxLAvJx91PAQO~1vXGshrEUw8sSfDerSt~yx-jJwGCSrjYN11AfcNNKRXrbX13dC6cSkZlrFB~zhFfvTKmGWFSVW2-Fw4AouGr0bnSDQGgxO~2OMoBX3eNRHQ~Gu3R9gHEGEXHk1Di~Y9bLUTFohWc1jAezgki0DzEAk01N2zX4l1Xjmt~klIT7oFeioMQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of thrombin on CD45 and Lck reconstituted deficient clones. (A) Thrombin-induced [Ca2+]i responses. JE6.1 (□), J45.01 (⊞), J45.01/CD45 (⧫), JCaM1 (▴), or JCaM1/Lck (◊) were stimulated with 100 nmol/L thrombin and fluorescence analyzed as a function of time. (B and C) Thrombin-mediated phosphorylation of p38MAPK. Cells were stimulated with 100 nmol/L thrombin for the times indicated. Proteins from cell lysates were subjected to SDS-PAGE as described in the legend to Fig 4 and were transferred to Immobilon membranes for Western blotting using phospho-specific p38 MAPK antibody. Equal amounts of p38 MAPK were detected in each condition (not shown).
