Effect of the caspase 3 inhibitor Ac-DEVD-CHO on GW1843-generated apoptotic DNA. (Left) Log-phase Molt-4 cells were treated for 48 hours with GW1843 alone or in combination with 50 μmol/L Ac-DEVD-CHO. Cells were harvested, DNA fragments isolated, and resolved using agarose gel electrophoresis as described in Materials and Methods. Lanes A, B, and C were treated with 0, 8, and 16 nmol/L GW1843, respectively. Lanes D, E, and F were treated with 0, 8, and 16 nmol/L GW1843, respectively, in the presence of 50 μmol/L Ac-DEVD-CHO. This gel is from a representative experiment from three separate determinations. (Right) Log-phase Molt-4 cells were treated for 48 hours as described below and prepared for FACS analysis as described in Materials and Methods. Cells were treated as follows: (A), mock treated cells; (B), 50 μmol/L Ac-DEVD-CHO; (C), 8 nmol/L GW1843; and (D), 8 nmol/L GW1843 with 50 μmol/L Ac-DEVD-CHO.