American Society of Hematology

Fig. 7.

$Fig. 7. Western blot of neutrophil cell fractions. Lane M corresponds to prestained molecular weight markers of 116, 80, 49, and 32 kD. Lane 1 corresponds to purified NAMLAA from human plasma. Lanes 2, 3, 4, and 5 correspond to the azurophilic granule–containing fraction, the cytosol fraction, the specific granule–containing fraction, and the purified plasma membrane fraction, respectively. Only the azurophilic granule–containing fraction and the membrane fraction were recognized by MoAb AAA4, producing a band with a lower molecular mass than the NAMLAA purified from plasma in lane 1.$

Western blot of neutrophil cell fractions. Lane M corresponds to prestained molecular weight markers of 116, 80, 49, and 32 kD. Lane 1 corresponds to purified NAMLAA from human plasma. Lanes 2, 3, 4, and 5 correspond to the azurophilic granule–containing fraction, the cytosol fraction, the specific granule–containing fraction, and the purified plasma membrane fraction, respectively. Only the azurophilic granule–containing fraction and the membrane fraction were recognized by MoAb AAA4, producing a band with a lower molecular mass than the NAMLAA purified from plasma in lane 1.

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