RNase protection analysis using an antisense riboprobe (420 bases) from the 5′ end of the CLMF cDNA results in smaller protected fragments of 293-297 bases from induced monocyte mRNA. RNA was isolated from VDS cells (VDS) or from monocytes stimulated as indicated with nothing, IFN-γ, or LPS, or both (sequentially). YRNA, yeast RNA control. RNA was hybridized overnight with a³²P-labeled antisense RNA probe transcribed from theEcoRV site at base 388 of the CLMF p35 cDNA. Products were treated with RNase T1, extracted, precipitated, and loaded onto a 6% sequencing gel with in vitro transcribed Century markers (Ambion). Marker size is indicated in bases. The arrows indicate the two specific bands of 293 and 297 bases protected at the site labeled in Fig 1 as S1.