5′ RACE analysis of monocyte RNA yields a single amplification product, not detectable from VDS RNA, that maps to a unique transcription start site. Polyadenylated RNA was prepared from PMA-treated VDS cells or from IFN-γ–primed and LPS-stimulated monocytes and used to prepare a cDNA library according to manufacturer's recommendations (Marathon cDNA Amplification Kit; Clontech). Each library (VDS, lanes 1 and 2; monocyte, lanes 3 and 4) was amplified using the Marathon adaptor primer and two different gene-specific primers, GSP34 (lanes 1 and 3) and GSP50 (lanes 2 and 4). RACE products were resolved on a 1.5% agarose gel with ethidium bromide. Markers are multiples of 100 bp (Life Technologies).