Fig. 2.
Fig. 2. Altered dimerization of BAXG67R and BAXG108V in vivo compared with wild-type BAX. (A) Immunoprecipitation of BCL-2 from 0.25% NP-40 lysates of35S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAXG67R ,or HA-BAXG108V stably transfected clones using antihuman BCL-2 MoAb 6C8 and SDS-PAGE.3 (B) Immunoprecipitation and sodium dodecyl sulfate-PAGE analysis using anti-HA MoAb 12CA5 from 0.25% NP-40 lysates of 35S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAXG67R, or HA-BAXG108V stably transfected clones.

Altered dimerization of BAXG67R and BAXG108V in vivo compared with wild-type BAX. (A) Immunoprecipitation of BCL-2 from 0.25% NP-40 lysates of35S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAXG67R ,or HA-BAXG108V stably transfected clones using antihuman BCL-2 MoAb 6C8 and SDS-PAGE.3 (B) Immunoprecipitation and sodium dodecyl sulfate-PAGE analysis using anti-HA MoAb 12CA5 from 0.25% NP-40 lysates of 35S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAXG67R, or HA-BAXG108V stably transfected clones.

Close Modal

or Create an Account

Close Modal
Close Modal